Everything you need to start Monocyte Activation Testing.
Most sites that carry-out typical bacterial endotoxin testing will likely already have all the facilities in their cell culture lab necessary to start monocyte activation testing too. Besides our CTL-MAT kit which contains all the specific MAT reagents you’ll need, the list below includes all further equipment and apparatus required at your site to get the ball rolling, in one neat overview.
Monocyte activation testing equipment
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Biological safety cabinet
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Humidified incubator (37°C, 5% CO2)
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Water bath (37°C)
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Vortex mixer
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Electronic Handystep
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Single and multi-channel adjustable volume pipettes suitable for 5 to 1000 μl (electronic recommended)
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Pipette controller (PIPETBOY) 0,1 to 100 ml
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Microplate shaker, multiple plates
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ELISA washer
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm
Sterile and pyrogen-free materials required for monocyte activation testing
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Repeat dispenser tips 5 ml
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Handystep tips 5 ml
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50 ml flacons of conical centrifuge tubes
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Disposable serological pipettes 1, 2, 5 and 10 ml
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Pipette tips 5 to 1.000 ml
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Eppendorf microcentrifuge 2 ml tubes, or 6, 24 or 32-well flat bottom plates (Nunc, to be used for making pre-dilutions of products and standard)
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96 well round/flat bottom, clear microplates
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Flasks, beakers and liquid containers necessary for preparation of reagents
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Multichannel micropipette reservoir
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Sterile gauze pads
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FACS tubes 2 ml
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FACS tubes holder
Supporting monocyte activation test reagents
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Isopropyl alcohol (70%)
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Deionized water
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Distilled water
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Phosphate buffered saline (PBS), sterile